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Etiology of wilt and basal rot of Jatropha curcas in Arriaga, Chiapas, Mexico. Salazar-Pinacho 1. Rosales-Esquinca 1. In the municipality of Arriaga, Chiapas, the death of physic nut Jatropha curcas L. The objective of this study was to determine the causal agent of the physic nut death. The isolates obtained were purified and subjected to biochemical and hypersensitivity tests on tobacco Nicotiana tabacum cv. The identification was positive for three isolates of Ralstonia solanacearum. However, it was not possible to complete Koch's postulates; therefore, R.
The physic nut Jatropha curcas L. In Arriaga, Chiapas, since , commercial plantations have been observed to contain dead 1- and 2-year old physic nut plants, which presented symptoms of wilting and basal stem rot. The aim of this study was to determine the etiology of the wilting and basal stem rot in the physic nut plant in Arriaga, Chiapas.
The samples were processed and analyzed under laboratory conditions. Different tests were carried out with pure cultures, including: the hypersensitivity reaction test, with the infiltration of a bacterial solution at 1. Xanthi leaves from day old plants. Concentration was calculated using the McFarland scale , a test of rotting in potato tubercula of the cv. The plants gathered from commercial fields displayed symptoms of wilting on leaves, bacterial rot on the base of the stem and root.
This corresponds with the description of symptoms reported by Hendroko et al. The incidence of the disease fell noticeably when the crop reached its third year of age. From the samples gathered on the field, 16 strains were obtained, 11 of which were discarded using the test of hypersensitivity on tobacco and the biochemical tests applied on it. Strains 1 and 3B showed positive results on the hypersensitivity test on tobacco after 48 h Figure 1 , and strain P3 also showed positive results or variables after 72 h; in this sense, Ji et al.
Finally, negative results were obtained for this test with strains P1 and P2. Xanthi, performed on the isolated strain 1 of physic nut. The five isolated bacterial strains from physic nut were positive in a TTC media, with the growth of colonies colored red with white edges, distinctive features of colonies infected with R. These results coincide with reports by Perea et al.
The Gram staining and biochemical tests for oxidase, catalase, urease, coincide with reports by Yabuuchi et al. Of Plant Disease, Dept. The wilting and basal rotting of Jatropha curcas in Arriaga, Chiapas, Mexico are characterized by symptoms of flabbiness in petioles, wilting and loss of leaves, rotting in the root and base of the stem, production of adventitious roots and senescence 20 to 30 days after the first symptoms.
Out of 16 isolated bacterial strains in physic nut plants that underwent the tobacco hypersensitivity test, three strains coincide with the characteristics for R. Although Koch's postulates were not fulfilled, this is the first report on the relation of Ralstonia solanacearum in wilting and basal rotting of the physic nut plant in Chiapas, Mexico. Laboratory guide for identification of plant pathogenic bacteria. Third Edition.
American Phytopathological Society Press. Characteristics of Ralstonia solanacearum strains of potato wilt disease from Nepal and Thailand.
Nepal Agriculture Research Journal European and Mediterranean Plant Protection Organization. EPPO Bulletin Ginting C and Maryono T. Physic nut Jatropha curcas L. Biotropia 16 1 The attack of disease occurred at ETF Jatropha seed garden. Cikarang, Indonesia. Hidayah N and Yulianti T.
Development of bacterial wilt of physic nut Jatropha curcas L. Proceedings of the Third National Workshop on Jatropha. Malang, East Java, Indonesia. New diversity of Ralstonia solanacearum strains associated with vegetable and ornamental crops in Florida. Plant Disease Characterization of isolates of Ralstonia solanacearum biovar2, pathogenic to Eucalyptus "urograndis" hybrids. Tropical Plant Pathology 37 6 McFarland J. The nephelometer: an instrument for estimating the numbers of bacteria in suspensions used for calculating the opsonic index and for vaccines.
Journal of the American Medical Association 49 14 Biochemical characterization of Ralstonia solanacerum causing bacterial wilt of brinjal in Bangladesh.
Progressive Agriculture 21 Transfer of two Burkholderia and an Alcaligenes species to Ralstonia gen. Microbiology and Immunology Jatropha curcas L. Similares em SciELO. Phytopathological notes Etiology of wilt and basal rot of Jatropha curcas in Arriaga, Chiapas, Mexico. Received: July 30, ; Accepted: November 16, Como citar este artigo.
Production of cytotoxic compounds in dedifferentiated cells of Jatropha curcas L. (Euphorbiaceae).
Etiology of wilt and basal rot of Jatropha curcas in Arriaga, Chiapas, Mexico. Salazar-Pinacho 1. Rosales-Esquinca 1. In the municipality of Arriaga, Chiapas, the death of physic nut Jatropha curcas L. The objective of this study was to determine the causal agent of the physic nut death.
Jatropha curcas L. Jatropha , a shrub species of the family Euphorbiaceae, has been recognized as a promising biofuel plant for reducing greenhouse gas emissions. However, recent attempts at commercial cultivation in Africa and Asia have failed because of low productivity. It is important to elucidate genetic diversity and relationship in worldwide Jatropha genetic resources for breeding of better commercial cultivars. Here, genetic diversity was analyzed by using accessions from Mesoamerica, Africa and Asia, based on 59 simple sequence repeat markers and eight retrotransposon-based insertion polymorphism markers.